Single-tube multiplex real-time PCR assay for rapid and reliable detection of HLA-A?31:01 allele

Author(s):

Zhang, T.; Xiao, Y.; Wang, Y.; Li, Y.; Zhang, L.; Chen, C.; Wang, H.

Format:

Journal article

Citation:

Pharmacogenomics, Volume 19, Issue 10 (2018). pp. 837-846.

Language:

English

Abstract:

Aim: HLA-A?31:01 has been associated with carbamazepine-induced hypersensitivity reactions. HLA-A?31:01 genetic testing is recommended before the initiation of carbamazepine therapy. Methods: A novel real-time PCR assay was designed for HLA-A?31:01 detection by allele-specific primers and TaqMan minor groove binding probes. Results: The genotyping results in 100 subjects by the established method who were in 100% agreement with the sequencing-based typing method. The assay presents a sensitivity of 1 (95% CI: 0.69-1.00), a specificity of 1 (95% CI: 0.96-1.00) and a positive and negative predictive value of 1. The carrier rates of HLA-A?31:01 in Tibetan (n = 45), Han Chinese (n = 100), Miaos (n = 48) and Khalkhas (n = 48) were 22.2, 10, 4.2 and 18.8%, respectively. Conclusion: This assay is reliable to detect HLA-A?31:01 and would be useful to prevent carbamazepine-induced hypersensitivity reactions. © 2018 Future Medicine Ltd.

ISSN:

14622416 (ISSN)

DOI:

10.2217/pgs-2018-0050

Identifier:

HmongStudies3915