Sero- and molecular typing of Duffy blood group in Southeast Asians and Oceanians
Author(s):
Shimizu, Y.; Ao, H.; Soemantri, A.; Tiwawech, D.; Settweetham-ishida, W.; Kayame, O. W.; Kimura, M.; Nishioka, T.; Ishida, T.
Format:
Journal article
Citation:
Human Biology, Volume 72, Issue 3 (2000-06). pp. 511-518.
Language:
English
Abstract:
Duffy blood group was studied among malaria-endemic Thai and Indonesian populations: Hmong (n = 103), Akha (n = 218), Lisu (n = 44), Bugis (n = 95), Toraja (n = 77), Dani (n = 44), Mee (n = 80) and Irianese (n = 81). Phenotypes were studied by the ordinal indirect Coombs' test and genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test. DNA analysis was used to type the Duffy blood group system. Deduced allele frequencies of Fy(a) (0.95 similar to 1.0) based on the phenotypes were similar to those in other populations in Southeast Asia and Oceania. The study revealed the presence of Fya-antigens showing weak reactivity to antisera as well as a discrepancy between the genotype shown by the PCR-RFLP study and that predicted by the phenotype. The PCR-RFLP study also suggested the presence of an alternative genetic basis for the Fy(a - b -) phenotype, which differs from the African type.