Characterization of multi-drug resistant Mycobacterium tuberculosis from immigrants residing in the USA using Ion Torrent full-gene sequencing

Author(s):

Daum, L.T.; Fischer, G.W.; Sromek, J.; Khubbar, M.; Hunter, P.; Gradus, M.S.; Bhattacharyya, S.

Format:

Journal article

Citation:

Epidemiology And Infection, Volume 142, Issue 6 (2014). pp. 1328-1333.

Language:

English

Abstract:

Drug-resistant Mycobacterium tuberculosis bacterium (MTB) is spreading worldwide. Three drug-resistant isolates were detected in Burmese, Hmong, and Indian immigrants currently residing in Milwaukee, Wisconsin, USA. Ion Torrent full-gene sequencing and complete genetic analysis was performed within 5 days and compared to results from traditional drug sensitivity testing (DST). Genetic characterization of seven, full-length resistance-Associated genes revealed two MDR and one highly resistant strain with important drug-resistant mutations that were confirmed by traditional DST. The rapid turnaround from sample-to-sequence underscores the public health value of Ion Torrent full-gene sequencing of MDR/XDR genes from epidemiologically significant clinical isolates. Copyright © Cambridge University Press 2013 The online version of this article is published within an Open Access environment subject to the conditions of the Creative Commons Attribution-NonCommercial-ShareAlike licence <http://creativecommons.org/licenses/by-nc-sa/3.0/>. The written permission of Cambridge University Press must be obtained for commercial re-use. © 2013 Cambridge University Press.

ISSN:

09502688 (ISSN)

DOI:

10.1017/S0950268813002409

Identifier:

HmongStudies0661